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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
25/09/2016 |
Actualizado : |
09/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
ARRUABARRENA, A.; BENITEZ-GALEANO, M.J.; GIAMBIASI, M.; BERTALMIO, A.; COLINA, R.; HERNÁNDEZ-RODRÍGUEZ, L. |
Afiliación : |
ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA JOSÉ BENÍTEZ-GALEANO, Laboratorio de Virología Molecular, Centro Universitario Regional Noroeste (CENUR Noroeste), Universidad de la República; MARIO ALEJANDRO GIAMBIASI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA MARIA BERTALMIO CASARIEGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODNEY COLINA, Laboratorio de Virología Molecular, Centro Universitario Regional Noroeste (CENUR Noroeste), Universidad de la República; LESTER HERNÁNDEZ-RODRÍGUEZ, Instituto de Investigaciones en Fruticultura Tropical, La Habana, Cuba. |
Título : |
Application of a simple and affordable protocol for isolating plant total nucleic acids for RNA and DNA virus detection. |
Fecha de publicación : |
2016 |
Fuente / Imprenta : |
Journal of Virological Methods, 2016, v.237, p. 14-17. |
DOI : |
10.1016/j.jviromet.2016.08.011 |
Idioma : |
Inglés |
Notas : |
Article history: Received 30 May 2016 / Received in revised form 26 July 2016 / Accepted 14 August 2016 / Available online 16 August 2016. |
Contenido : |
ABSTRACT.
Standard molecular methods for plant virus diagnosis require the purification of RNA or DNA extracts from a large number of samples, with sufficient concentration and quality for their use in PCR, RT-PCR, or qPCR analysis. Most methods are laborious and use either hazardous and/or costly chemicals.Apreviously published protocol for RNA isolation from several plant species yields high amounts of good quality RNADNA mixture in a simple, safe and inexpensive manner. In the present work, this method was tested to obtain RNA-DNA extracts from leaves of tomato, potato and three species of citrus, and was compared with two commercial kits. The results demonstrated that this protocol offers at least comparable nucleic
acid quality, quantity and purity to those provided by commercial phenol-based or spin column systems and that are suitable to be used in PCR, RT-PCR and qPCR for virus and viroid detection. Because of its easy implementation and the use of safe and inexpensive reagents, it can be easily implemented to work in plant virus and viroid detection in different plant species.
© 2016 Elsevier B.V. All rights reserved |
Palabras claves : |
DNA; PCR; PLANT VIROID; PLANT VIRUS; PURIFICATION; RNA; RT-PCR. |
Thesagro : |
CITRUS. |
Asunto categoría : |
-- |
Marc : |
LEADER 02137naa a2200301 a 4500 001 1055729 005 2019-10-09 008 2016 bl uuuu u00u1 u #d 024 7 $a10.1016/j.jviromet.2016.08.011$2DOI 100 1 $aARRUABARRENA, A. 245 $aApplication of a simple and affordable protocol for isolating plant total nucleic acids for RNA and DNA virus detection.$h[electronic resource] 260 $c2016 500 $aArticle history: Received 30 May 2016 / Received in revised form 26 July 2016 / Accepted 14 August 2016 / Available online 16 August 2016. 520 $aABSTRACT. Standard molecular methods for plant virus diagnosis require the purification of RNA or DNA extracts from a large number of samples, with sufficient concentration and quality for their use in PCR, RT-PCR, or qPCR analysis. Most methods are laborious and use either hazardous and/or costly chemicals.Apreviously published protocol for RNA isolation from several plant species yields high amounts of good quality RNADNA mixture in a simple, safe and inexpensive manner. In the present work, this method was tested to obtain RNA-DNA extracts from leaves of tomato, potato and three species of citrus, and was compared with two commercial kits. The results demonstrated that this protocol offers at least comparable nucleic acid quality, quantity and purity to those provided by commercial phenol-based or spin column systems and that are suitable to be used in PCR, RT-PCR and qPCR for virus and viroid detection. Because of its easy implementation and the use of safe and inexpensive reagents, it can be easily implemented to work in plant virus and viroid detection in different plant species. © 2016 Elsevier B.V. All rights reserved 650 $aCITRUS 653 $aDNA 653 $aPCR 653 $aPLANT VIROID 653 $aPLANT VIRUS 653 $aPURIFICATION 653 $aRNA 653 $aRT-PCR 700 1 $aBENITEZ-GALEANO, M.J. 700 1 $aGIAMBIASI, M. 700 1 $aBERTALMIO, A. 700 1 $aCOLINA, R. 700 1 $aHERNÁNDEZ-RODRÍGUEZ, L. 773 $tJournal of Virological Methods, 2016$gv.237, p. 14-17.
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INIA Las Brujas (LB) |
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
21/02/2014 |
Actualizado : |
06/11/2018 |
Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
Autor : |
GARCÍA PETILLO, M. |
Afiliación : |
MARIO WELLS GARCIA PETILLO. |
Título : |
Conceptos básicos para el diseño y el manejo del riego ; [capítulo] 2. |
Fecha de publicación : |
2012 |
Fuente / Imprenta : |
In: Grupo de Desarrollo de Riego; Seminario Internacional, 2do., 2012, Salto, Uruguay. Riego en cultivos y pasturas. Montevideo (Uruguay): INIA, 2012. |
Páginas : |
p. 3-4. |
ISBN : |
978-9974-38-356-2 |
Idioma : |
Español |
Notas : |
Bibliografía: p. 113. |
Palabras claves : |
AGUA DISPONIBLE; AGUA FÁCILMENTE DISPONIBLE; CAPACIDAD DE CAMPO; CAUDAL DE RIEGO; COEFICIENTE DE CULTIVO; EVAPOTRANSPIRACIÓN DEL CULTIVO; FRECUENCIA DE RIEGO; LÁMINA BRUTA; LÁMINA NETA; PUNTO DE MARCHITEZ PERMANENTE; UMBRAL DE RIEGO. |
Thesagro : |
SISTEMAS DE RIEGO. |
Asunto categoría : |
F06 Riego |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/11843/1/Garcia-M.-2012.pdf
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Marc : |
LEADER 00970naa a2200289 a 4500 001 1048284 005 2018-11-06 008 2012 bl uuuu u00u1 u #d 020 $a978-9974-38-356-2 100 1 $aGARCÍA PETILLO, M. 245 $aConceptos básicos para el diseño y el manejo del riego ; [capítulo] 2. 260 $c2012 300 $ap. 3-4. 500 $aBibliografía: p. 113. 650 $aSISTEMAS DE RIEGO 653 $aAGUA DISPONIBLE 653 $aAGUA FÁCILMENTE DISPONIBLE 653 $aCAPACIDAD DE CAMPO 653 $aCAUDAL DE RIEGO 653 $aCOEFICIENTE DE CULTIVO 653 $aEVAPOTRANSPIRACIÓN DEL CULTIVO 653 $aFRECUENCIA DE RIEGO 653 $aLÁMINA BRUTA 653 $aLÁMINA NETA 653 $aPUNTO DE MARCHITEZ PERMANENTE 653 $aUMBRAL DE RIEGO 773 $tIn: Grupo de Desarrollo de Riego; Seminario Internacional, 2do., 2012, Salto, Uruguay. Riego en cultivos y pasturas. Montevideo (Uruguay): INIA, 2012.
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